diff --git a/charts/karmada/README.md b/charts/karmada/README.md
index 1a7aa278e..1489ae199 100644
--- a/charts/karmada/README.md
+++ b/charts/karmada/README.md
@@ -196,7 +196,7 @@ helm install karmada-scheduler-estimator -n karmada-system ./charts/karmada
 
 
 | Name                                     | Description                                                                                                                                                                                                                                   | Value                                                                                                                                                                                                                |
-| ---------------------------------------- | --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- |
+| ---------------------------------------- |-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------| -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- |
 | `installMode`                            | InstallMode "host", "agent" and "component" are provided, "host" means install karmada in the control-cluster, "agent" means install agent client in the member cluster, "component" means install selected components in the control-cluster | `"host"`                                                                                                                                                                                                             |
 | `clusterDomain`                          | Default cluster domain for karmada                                                                                                                                                                                                            | `"cluster.local"`                                                                                                                                                                                                    |
 | `components`                             | Selected components list, selectable values: "schedulerEstimator"                                                                                                                                                                             | `[]`                                                                                                                                                                                                                 |
@@ -233,6 +233,7 @@ helm install karmada-scheduler-estimator -n karmada-system ./charts/karmada
 | `etcd.internal.pvc.size`                 | Storage size of the etcd, takes effect when`etcd.internal.storageType` is "pvc"                                                                                                                                                               | `""`                                                                                                                                                                                                                 |
 | `etcd.internal.nodeSelector`             | Node selector of the karmada-apiserver                                                                                                                                                                                                        | `{}`                                                                                                                                                                                                                 |
 | `etcd.internal.resources`                | Resource quota of the etcd                                                                                                                                                                                                                    | `{}`                                                                                                                                                                                                                 |
+| `etcd.internal.tolerations`              | Tolerations of the internal etcd                                                                                                                                                                                                              | `[]`                                                                                                                                                                                                                 |
 | `agent.clusterName`                      | Name of the member cluster                                                                                                                                                                                                                    | `""`                                                                                                                                                                                                                 |
 | `agent.clusterEndpoint`                  | Server endpoint of the member cluster                                                                                                                                                                                                         | `""`                                                                                                                                                                                                                 |
 | `agent.kubeconfig.caCrt`                 | CA CRT of the karmada certificate                                                                                                                                                                                                             | `""`                                                                                                                                                                                                                 |
@@ -293,9 +294,9 @@ helm install karmada-scheduler-estimator -n karmada-system ./charts/karmada
 | `controllerManager.strategy`             | Strategy of the karmada-controller-manager                                                                                                                                                                                                    | `{"type": "RollingUpdate", "rollingUpdate": {"maxUnavailable": "0", "maxSurge": "50%"} }`                                                                                                                            |
 | `controllerManager.featureGates`         | FeatureGates of the karmada-controller-manager                                                                                                                                                                                                | `{"PropagateDeps": "false"}`                                                                                                                                                                                         |
 | `controllerManager.controllers`          | Controllers of the karmada-controller-manager                                                                                                                                                                                                 | `""`                                                                                                                                                                                                                 |
-| `controllerManager.extraCommandArgs`          | extra command args of the karmada-controller-manager                                                                                                                                                                                                 | `{}`                                                                                                                                                                                                                 |
+| `controllerManager.extraCommandArgs`     | extra command args of the karmada-controller-manager                                                                                                                                                                                          | `{}`                                                                                                                                                                                                                 |
 | `apiServer.labels`                       | Labels of the karmada-apiserver deployment                                                                                                                                                                                                    | `{"app": "karmada-apiserver"}`                                                                                                                                                                                       |
-| `apiServer.serviceAnnotations`           | Annotations of the karmada-apiserver service                                                                                                                                                                                                    | `{}`                                                                                                                                                                                       |
+| `apiServer.serviceAnnotations`           | Annotations of the karmada-apiserver service                                                                                                                                                                                                  | `{}`                                                                                                                                                                                       |
 | `apiServer.replicaCount`                 | Target replicas of the karmada-apiserver                                                                                                                                                                                                      | `1`                                                                                                                                                                                                                  |
 | `apiServer.podLabels`                    | Labels of the karmada-apiserver pods                                                                                                                                                                                                          | `{}`                                                                                                                                                                                                                 |
 | `apiServer.podAnnotations`               | Annotations of the karmada-apiserver pods                                                                                                                                                                                                     | `{}`                                                                                                                                                                                                                 |
diff --git a/charts/karmada/templates/etcd.yaml b/charts/karmada/templates/etcd.yaml
index 985c8e05d..de4e61a58 100644
--- a/charts/karmada/templates/etcd.yaml
+++ b/charts/karmada/templates/etcd.yaml
@@ -39,8 +39,10 @@ spec:
       nodeSelector:
       {{- toYaml . | nindent 8 }}
       {{- end }}
+      {{- with .Values.etcd.internal.tolerations }}
       tolerations:
-        - operator: Exists
+      {{- toYaml . | nindent 8 }}
+      {{- end }}
       containers:
         - name: etcd
           image: {{ template "karmada.internal.etcd.image" . }}
diff --git a/charts/karmada/values.yaml b/charts/karmada/values.yaml
index d4403dee1..4a141d5c5 100644
--- a/charts/karmada/values.yaml
+++ b/charts/karmada/values.yaml
@@ -578,6 +578,10 @@ etcd:
       size: ""
     ## @param etcd.internal.nodeSelector node selector of the etcd
     nodeSelector: {}
+    ## @param etcd.internal.tolerations tolerations of the internal etcd
+    tolerations: []
+      # - key: node-role.kubernetes.io/master
+      #   operator: Exists
     ## @param etcd.internal.resources
     resources: {}
       # If you do want to specify resources, uncomment the following