diff --git a/defaults.env b/defaults.env
index 204ac1fce..9fdb11118 100644
--- a/defaults.env
+++ b/defaults.env
@@ -2,5 +2,5 @@ export CORECLR_ENABLE_PROFILING=1
 export CORECLR_PROFILER='{918728DD-259F-4A6A-AC2B-B85E1B658318}'
 export CORECLR_PROFILER_PATH=/opt/opentelemetry-dotnet-autoinstrumentation/OpenTelemetry.ClrProfiler.Native.so
 export DOTNET_STARTUP_HOOKS=/opt/opentelemetry-dotnet-autoinstrumentation/OpenTelemetry.Instrumentation.StartupHook.dll
-export OTEL_INTEGRATIONS=/opt/opentelemetry-dotnet-autoinstrumentation/integrations.json
-export OTEL_DOTNET_TRACER_HOME=/opt/opentelemetry-dotnet-autoinstrumentation
\ No newline at end of file
+export OTEL_DOTNET_AUTO_INTEGRATIONS_FILE=/opt/opentelemetry-dotnet-autoinstrumentation/integrations.json
+export OTEL_DOTNET_AUTO_HOME=/opt/opentelemetry-dotnet-autoinstrumentation
\ No newline at end of file
diff --git a/dev/envvars.sh b/dev/envvars.sh
index 7eac896b9..97cfaf7d0 100644
--- a/dev/envvars.sh
+++ b/dev/envvars.sh
@@ -56,9 +56,8 @@ fi
 export DOTNET_STARTUP_HOOKS="${CURDIR}/bin/tracer-home/netcoreapp3.1/OpenTelemetry.Instrumentation.StartupHook.dll"
 
 # Configure OpenTelemetry Tracer 
-export OTEL_DOTNET_TRACER_HOME="${CURDIR}/bin/tracer-home"
-export OTEL_INTEGRATIONS="${CURDIR}/bin/tracer-home/integrations.json"
-export OTEL_TRACE_DEBUG="1"
-export OTEL_DUMP_ILREWRITE_ENABLED="0"
-export OTEL_CLR_ENABLE_INLINING="1"
-export OTEL_PROFILER_EXCLUDE_PROCESSES="dotnet.exe,dotnet"
+export OTEL_DOTNET_AUTO_HOME="${CURDIR}/bin/tracer-home"
+export OTEL_DOTNET_AUTO_INTEGRATIONS_FILE="${CURDIR}/bin/tracer-home/integrations.json"
+export OTEL_DOTNET_AUTO_DEBUG="1"
+export OTEL_DOTNET_AUTO_DUMP_ILREWRITE_ENABLED="0"
+export OTEL_DOTNET_AUTO_EXCLUDE_PROCESSES="dotnet.exe,dotnet"
diff --git a/devenv.bat b/devenv.bat
index 966f1c00a..8ca06d27f 100644
--- a/devenv.bat
+++ b/devenv.bat
@@ -48,11 +48,11 @@ SET CORECLR_PROFILER={918728DD-259F-4A6A-AC2B-B85E1B658318}
 SET CORECLR_PROFILER_PATH=%~dp0\src\OpenTelemetry.ClrProfiler.Native\bin\%profiler_configuration%\%profiler_platform%\OpenTelemetry.ClrProfiler.Native.dll
 
 rem Don't attach the profiler to these processes
-SET OTEL_PROFILER_EXCLUDE_PROCESSES=devenv.exe;Microsoft.ServiceHub.Controller.exe;ServiceHub.Host.CLR.exe;ServiceHub.TestWindowStoreHost.exe;ServiceHub.DataWarehouseHost.exe;sqlservr.exe;VBCSCompiler.exe;iisexpresstray.exe;msvsmon.exe;PerfWatson2.exe;ServiceHub.IdentityHost.exe;ServiceHub.VSDetouredHost.exe;ServiceHub.SettingsHost.exe;ServiceHub.Host.CLR.x86.exe;vstest.console.exe;ServiceHub.RoslynCodeAnalysisService32.exe;testhost.x86.exe;MSBuild.exe;ServiceHub.ThreadedWaitDialog.exe
+SET OTEL_DOTNET_AUTO_EXCLUDE_PROCESSES=devenv.exe;Microsoft.ServiceHub.Controller.exe;ServiceHub.Host.CLR.exe;ServiceHub.TestWindowStoreHost.exe;ServiceHub.DataWarehouseHost.exe;sqlservr.exe;VBCSCompiler.exe;iisexpresstray.exe;msvsmon.exe;PerfWatson2.exe;ServiceHub.IdentityHost.exe;ServiceHub.VSDetouredHost.exe;ServiceHub.SettingsHost.exe;ServiceHub.Host.CLR.x86.exe;vstest.console.exe;ServiceHub.RoslynCodeAnalysisService32.exe;testhost.x86.exe;MSBuild.exe;ServiceHub.ThreadedWaitDialog.exe
 
 rem Set dotnet tracer home path
-SET OTEL_DOTNET_TRACER_HOME=%~dp0
-SET OTEL_INTEGRATIONS=%OTEL_DOTNET_TRACER_HOME%\integrations.json
+SET OTEL_DOTNET_AUTO_HOME=%~dp0
+SET OTEL_DOTNET_AUTO_INTEGRATIONS_FILE=%OTEL_DOTNET_AUTO_HOME%\integrations.json
 
 if "%start_visual_studio%" == "true" (
     echo Starting Visual Studio...
diff --git a/docs/DESIGN.md b/docs/DESIGN.md
index b7ca610c4..8e8d6a291 100644
--- a/docs/DESIGN.md
+++ b/docs/DESIGN.md
@@ -59,7 +59,7 @@ injected into the application.
 contains the code setting up the OpenTelemtry .NET SDK and configured instrumentations.
 Plus, support code to run and implement bytecode instrumentations. Optionally, the code
 setting-up the OpenTelemetry .NET SDK can be left to the application by setting the
-environment variable `OTEL_DOTNET_TRACER_LOAD_AT_STARTUP` to `false`.
+environment variable `OTEL_DOTNET_AUTO_LOAD_AT_STARTUP` to `false`.
 
 - **Source Instrumentations**: instrumentations created on top of API hooks/callbacks provided
 directly by the library/framework being instrumented. This type of instrumentation depends on the
diff --git a/docs/USAGE.md b/docs/USAGE.md
index 939011b66..cf36045ef 100644
--- a/docs/USAGE.md
+++ b/docs/USAGE.md
@@ -8,10 +8,11 @@
 
 | Environment variable | Description | Default |
 |-|-|-|
-| `OTEL_TRACE_ENABLED` | Enable to activate the tracer. | `true` |
-| `OTEL_PROFILER_PROCESSES` | Sets the filename of executables the profiler can attach to. If not defined (default), the profiler will attach to any process. Supports multiple values separated with comma, for example: `MyApp.exe,dotnet.exe` |  |
-| `OTEL_PROFILER_EXCLUDE_PROCESSES` | Sets the filename of executables the profiler cannot attach to. If not defined (default), the profiler will attach to any process. Supports multiple values separated with comma, for example: `MyApp.exe,dotnet.exe` |  |
-| `OTEL_AZURE_APP_SERVICES` | Set to indicate that the profiler is running in the context of Azure App Services. | `false` |
+| `OTEL_DOTNET_AUTO_HOME` | Installation location. | `true` |
+| `OTEL_DOTNET_AUTO_ENABLED` | Enable to activate the tracer. | `true` |
+| `OTEL_DOTNET_AUTO_INCLUDE_PROCESSES` | Sets the filename of executables the profiler can attach to. If not defined (default), the profiler will attach to any process. Supports multiple values separated with comma, for example: `MyApp.exe,dotnet.exe` |  |
+| `OTEL_DOTNET_AUTO_EXCLUDE_PROCESSES` | Sets the filename of executables the profiler cannot attach to. If not defined (default), the profiler will attach to any process. Supports multiple values separated with comma, for example: `MyApp.exe,dotnet.exe` |  |
+| `OTEL_DOTNET_AUTO_AZURE_APP_SERVICES` | Set to indicate that the profiler is running in the context of Azure App Services. | `false` |
 
 ### Resource
 
@@ -28,14 +29,14 @@ for more details.
 
 | Environment variable | Description | Default |
 |-|-|-|
-| `OTEL_INTEGRATIONS` | The file path of bytecode instrumentations JSON configuration file. Usually it should be set to `%ProfilerDirectory%/integrations.json` | |
-| `OTEL_DOTNET_TRACER_INSTRUMENTATIONS` | The instrumentations you want to enable, separated by a comma. Supported values: `AspNet`, `HttpClient`, `SqlClient`, `MongoDb`. |  |
-| `OTEL_DOTNET_TRACER_DISABLED_INSTRUMENTATIONS` | The instrumentations set via `OTEL_DOTNET_TRACER_INSTRUMENTATIONS` value and `OTEL_INTEGRATIONS` configuration file you want to disable, separated by a comma. | |
-| `OTEL_TRACE_{0}_ENABLED` | Configuration pattern for enabling or disabling a specific bytecode. For example, in order to disable MongoDb instrumentation, set `OTEL_TRACE_MongoDb_ENABLED=false` | `true` |
-| `OTEL_TRACE_DOMAIN_NEUTRAL_INSTRUMENTATION` |  Sets whether to intercept method calls when the caller method is inside a domain-neutral assembly. This is recommended when instrumenting IIS applications. | `false` |
-| `OTEL_CLR_DISABLE_OPTIMIZATIONS` |  Set to `true` to disable all JIT optimizations. | `false` |
-| `OTEL_CLR_ENABLE_INLINING` | Set to `false` to disable JIT inlining. | `true` |
-| `OTEL_CLR_ENABLE_NGEN` | Set to `false` to disable NGEN images. | `true` |
+| `OTEL_DOTNET_AUTO_INTEGRATIONS_FILE` | The file path of bytecode instrumentations JSON configuration file. Usually it should be set to `%ProfilerDirectory%/integrations.json` | |
+| `OTEL_DOTNET_AUTO_ENABLED_INSTRUMENTATIONS` | The instrumentations you want to enable, separated by a comma. Supported values: `AspNet`, `HttpClient`, `SqlClient`, `MongoDb`. |  |
+| `OTEL_DOTNET_AUTO_DISABLED_INSTRUMENTATIONS` | The instrumentations set via `OTEL_DOTNET_AUTO_ENABLED_INSTRUMENTATIONS` value and `OTEL_DOTNET_AUTO_INTEGRATIONS_FILE` configuration file you want to disable, separated by a comma. | |
+| `OTEL_DOTNET_AUTO_{0}_ENABLED` | Configuration pattern for enabling or disabling a specific bytecode. For example, in order to disable MongoDb instrumentation, set `OTEL_TRACE_MongoDb_ENABLED=false` | `true` |
+| `OTEL_DOTNET_AUTO_DOMAIN_NEUTRAL_INSTRUMENTATION` |  Sets whether to intercept method calls when the caller method is inside a domain-neutral assembly. This is recommended when instrumenting IIS applications. | `false` |
+| `OTEL_DOTNET_AUTO_CLR_DISABLE_OPTIMIZATIONS` |  Set to `true` to disable all JIT optimizations. | `false` |
+| `OTEL_DOTNET_AUTO_CLR_ENABLE_INLINING` | Set to `false` to disable JIT inlining. | `true` |
+| `OTEL_DOTNET_AUTO_CLR_ENABLE_NGEN` | Set to `false` to disable NGEN images. | `true` |
 
 ### ASP.NET (.NET Framework) Instrumentation
 
@@ -53,11 +54,11 @@ Default logs directory paths are:
 
 | Environment variable | Description | Default |
 |-|-|-|
-| `OTEL_TRACE_LOG_DIRECTORY` | The directory of the .NET Tracer logs. Overrides the value in `OTEL_TRACE_LOG_PATH` if present. | _see above_ |
-| `OTEL_TRACE_LOG_PATH` | The path of the profiler log file. | _see above_ |
-| `OTEL_TRACE_DEBUG` | Enable to activate debugging mode for the tracer. | `false` |
-| `OTEL_DOTNET_TRACER_CONSOLE_EXPORTER_ENABLED` | Defines whether the console exporter is enabled or not. | `true` |
-| `OTEL_DUMP_ILREWRITE_ENABLED` | Allows the profiler to dump the IL original code and modification to the log. | `false` |
+| `OTEL_DOTNET_AUTO_LOG_DIRECTORY` | The directory of the .NET Tracer logs. Overrides the value in `OTEL_DOTNET_AUTO_LOG_PATH` if present. | _see above_ |
+| `OTEL_DOTNET_AUTO_LOG_PATH` | The path of the profiler log file. | _see above_ |
+| `OTEL_DOTNET_AUTO_DEBUG` | Enable to activate debugging mode for the tracer. | `false` |
+| `OTEL_DOTNET_AUTO_CONSOLE_EXPORTER_ENABLED` | Defines whether the console exporter is enabled or not. | `true` |
+| `OTEL_DOTNET_AUTO_DUMP_ILREWRITE_ENABLED` | Allows the profiler to dump the IL original code and modification to the log. | `false` |
 
 ### Exporters
 
@@ -96,22 +97,22 @@ The Batch Span Processor batches of finished spans before they are send by the e
 
 | Environment variable | Description | Default |
 |-|-|-|
-| `OTEL_DOTNET_TRACER_LOAD_AT_STARTUP` | Defines whether the tracer is created by the auto instrumentation library or not. | `true` |
-| `OTEL_DOTNET_TRACER_ADDITIONAL_SOURCES` | Comma separated list of additional `ActivitySource` names to be added to the tracer at the startup. |  |
-| `OTEL_DOTNET_TRACER_LEGACY_SOURCES` | Comma separated list of additional legacy source names to be added to the tracer at the startup. |  |
-| `OTEL_DOTNET_TRACER_INSTRUMENTATION_PLUGINS` | Colon (:) separated list of OTel SDK instrumentation plugins represented by `System.Type.AssemblyQualifiedName`. | |
+| `OTEL_DOTNET_AUTO_LOAD_AT_STARTUP` | Defines whether the tracer is created by the auto instrumentation library or not. | `true` |
+| `OTEL_DOTNET_AUTO_ADDITIONAL_SOURCES` | Comma separated list of additional `ActivitySource` names to be added to the tracer at the startup. |  |
+| `OTEL_DOTNET_AUTO_LEGACY_SOURCES` | Comma separated list of additional legacy source names to be added to the tracer at the startup. |  |
+| `OTEL_DOTNET_AUTO_INSTRUMENTATION_PLUGINS` | Colon (:) separated list of OTel SDK instrumentation plugins represented by `System.Type.AssemblyQualifiedName`. | |
 
-`OTEL_DOTNET_TRACER_LOAD_AT_STARTUP` should be set to `false` when application
+`OTEL_DOTNET_AUTO_LOAD_AT_STARTUP` should be set to `false` when application
 initializes OpenTelemetry .NET SDK Tracer on its own. This configuration can be
 used e.g. to just get the bytecode instrumentations.
 
-`OTEL_DOTNET_TRACER_ADDITIONAL_SOURCES` should be used to capture manually
+`OTEL_DOTNET_AUTO_ADDITIONAL_SOURCES` should be used to capture manually
 instrumented spans.
 
-`OTEL_DOTNET_TRACER_LEGACY_SOURCES` can be used to capture `Activity` objects
+`OTEL_DOTNET_AUTO_LEGACY_SOURCES` can be used to capture `Activity` objects
 created without using the `ActivitySource` API.
 
-`OTEL_DOTNET_TRACER_INSTRUMENTATION_PLUGINS` can be used to extend the
+`OTEL_DOTNET_AUTO_INSTRUMENTATION_PLUGINS` can be used to extend the
 configuration of the the OpenTelemetry .NET SDK Tracer. A plugin must be a
 non-static, non-abstract class which has a default constructor and a method
 with following signature:
@@ -180,7 +181,7 @@ cat /proc/version
 
 The default installation of auto-instrumentation enables tracing all .NET processes on the box.
 In the typical scenarios (dedicated VMs or containers), this is not a problem.
-Use the environment variables `OTEL_PROFILER_EXCLUDE_PROCESSES` and `OTEL_PROFILER_PROCESSES`
+Use the environment variables `OTEL_DOTNET_AUTO_EXCLUDE_PROCESSES` and `OTEL_DOTNET_AUTO_INCLUDE_PROCESSES`
 to include/exclude applications from the tracing auto-instrumentation.
 These are ";" delimited lists that control the inclusion/exclusion of processes.
 
@@ -201,10 +202,10 @@ you can also manually add binding redirection to [the `App.config` file](../samp
 If none of the suggestions above solves your issue, detailed logs are necessary.
 Follow the steps below to get the detailed logs from OpenTelemetry AutoInstrumentation for .NET:
 
-Set the environment variable `OTEL_TRACE_DEBUG` to `true` before the instrumented process starts.
+Set the environment variable `OTEL_DOTNET_AUTO_DEBUG` to `true` before the instrumented process starts.
 By default, the library writes the log files under the below predefined locations.
-If needed, change the default location by updating the environment variable `OTEL_TRACE_LOG_PATH` to an appropriate path.
+If needed, change the default location by updating the environment variable `OTEL_DOTNET_AUTO_LOG_PATH` to an appropriate path.
 On Linux, the default log location is `/var/log/opentelemetry/dotnet/`
 On Windows, the default log location is `%ProgramData%\\OpenTelemetry .NET AutoInstrumentation\logs\`
 Compress the whole folder to capture the multiple log files and send the compressed folder to us.
-After obtaining the logs, remember to remove the environment variable `OTEL_TRACE_DEBUG` to avoid unnecessary overhead.
+After obtaining the logs, remember to remove the environment variable `OTEL_DOTNET_AUTO_DEBUG` to avoid unnecessary overhead.
diff --git a/poc.sh b/poc.sh
index 564008d15..50e5a20ab 100755
--- a/poc.sh
+++ b/poc.sh
@@ -43,13 +43,13 @@ docker run -d --rm --name jaeger \
   jaegertracing/all-in-one:1.22
 
 # instrument and run HTTP server app in background
-export OTEL_DOTNET_TRACER_INSTRUMENTATION_PLUGINS="Samples.AspNetCoreMvc.OtelSdkPlugin, Samples.AspNetCoreMvc31, Version=1.0.0.0, Culture=neutral, PublicKeyToken=null:Vendor.Distro.Plugin, Vendor.Distro, Version=0.0.1.0, Culture=neutral, PublicKeyToken=null"
-./dev/instrument.sh OTEL_DOTNET_TRACER_INSTRUMENTATIONS="AspNet,SqlClient,MongoDb" OTEL_SERVICE_NAME="aspnet-server" ASPNETCORE_URLS="http://127.0.0.1:8080/" dotnet run --no-launch-profile -f $aspNetAppTargetFramework --project ./samples/Samples.AspNetCoreMvc31/Samples.AspNetCoreMvc31.csproj &
-unset OTEL_DOTNET_TRACER_INSTRUMENTATION_PLUGINS
+export OTEL_DOTNET_AUTO_INSTRUMENTATION_PLUGINS="Samples.AspNetCoreMvc.OtelSdkPlugin, Samples.AspNetCoreMvc31, Version=1.0.0.0, Culture=neutral, PublicKeyToken=null:Vendor.Distro.Plugin, Vendor.Distro, Version=0.0.1.0, Culture=neutral, PublicKeyToken=null"
+./dev/instrument.sh OTEL_DOTNET_AUTO_ENABLED_INSTRUMENTATIONS="AspNet,SqlClient,MongoDb" OTEL_SERVICE_NAME="aspnet-server" ASPNETCORE_URLS="http://127.0.0.1:8080/" dotnet run --no-launch-profile -f $aspNetAppTargetFramework --project ./samples/Samples.AspNetCoreMvc31/Samples.AspNetCoreMvc31.csproj &
+unset OTEL_DOTNET_AUTO_INSTRUMENTATION_PLUGINS
 ./dev/wait-local-port.sh 8080
 
 # instrument and run HTTP client app
-time ./dev/instrument.sh OTEL_DOTNET_TRACER_INSTRUMENTATIONS="HttpClient" OTEL_SERVICE_NAME="http-client" dotnet run --no-launch-profile -f $sampleAppTargetFramework --project ./samples/${sampleApp}/${sampleApp}.csproj
+time ./dev/instrument.sh OTEL_DOTNET_AUTO_ENABLED_INSTRUMENTATIONS="HttpClient" OTEL_SERVICE_NAME="http-client" dotnet run --no-launch-profile -f $sampleAppTargetFramework --project ./samples/${sampleApp}/${sampleApp}.csproj
 
 # verify if it works
 read -p "Check traces under: http://localhost:16686/search. Press enter to continue"
diff --git a/samples/Samples.AspNetCoreMvc31/Properties/launchSettings.json b/samples/Samples.AspNetCoreMvc31/Properties/launchSettings.json
index 4ece54f21..4b40af69d 100644
--- a/samples/Samples.AspNetCoreMvc31/Properties/launchSettings.json
+++ b/samples/Samples.AspNetCoreMvc31/Properties/launchSettings.json
@@ -19,8 +19,8 @@
         "CORECLR_PROFILER_PATH": "$(SolutionDir)bin\\tracer-home\\win-x64\\OpenTelemetry.ClrProfiler.Native.dll",
         "DOTNET_STARTUP_HOOKS": "$(SolutionDir)bin\\tracer-home\\netcoreapp3.1\\OpenTelemetry.Instrumentation.StartupHook.dll",
 
-        "OTEL_DOTNET_TRACER_HOME": "$(SolutionDir)bin\\tracer-home",
-        "OTEL_INTEGRATIONS": "$(SolutionDir)bin\\tracer-home\\integrations.json",
+        "OTEL_DOTNET_AUTO_HOME": "$(SolutionDir)bin\\tracer-home",
+        "OTEL_DOTNET_AUTO_INTEGRATIONS_FILE": "$(SolutionDir)bin\\tracer-home\\integrations.json",
         "OTEL_SERVICE_NAME": "Samples.AspNetCoreMvc31",
         "OTEL_TRACES_EXPORTER": "jaeger",
         "OTEL_EXPORTER_JAEGER_AGENT_HOST": "localhost",
@@ -40,8 +40,8 @@
         "CORECLR_PROFILER_PATH": "$(SolutionDir)bin\\tracer-home$(OutputPath)\\OpenTelemetry.ClrProfiler.Native.dll",
         "DOTNET_STARTUP_HOOKS": "$(SolutionDir)bin\\tracer-home\\netcoreapp3.1\\OpenTelemetry.Instrumentation.StartupHook.dll",
 
-        "OTEL_DOTNET_TRACER_HOME": "$(SolutionDir)bin\\tracer-home",
-        "OTEL_INTEGRATIONS": "$(SolutionDir)bin\\tracer-home\\integrations.json",
+        "OTEL_DOTNET_AUTO_HOME": "$(SolutionDir)bin\\tracer-home",
+        "OTEL_DOTNET_AUTO_INTEGRATIONS_FILE": "$(SolutionDir)bin\\tracer-home\\integrations.json",
 
         "OTEL_SERVICE_NAME": "Samples.AspNetCoreMvc31",
         "OTEL_TRACES_EXPORTER": "jaeger",
diff --git a/samples/Samples.StartupHook.AspNetCoreMvc31/Properties/launchSettings.json b/samples/Samples.StartupHook.AspNetCoreMvc31/Properties/launchSettings.json
index 59700a064..20deb3133 100644
--- a/samples/Samples.StartupHook.AspNetCoreMvc31/Properties/launchSettings.json
+++ b/samples/Samples.StartupHook.AspNetCoreMvc31/Properties/launchSettings.json
@@ -21,7 +21,7 @@
 
         "OTEL_SERVICE_NAME": "Samples.StartupHook.AspNetCoreMvc31",
 
-        "OTEL_DOTNET_TRACER_INSTRUMENTATIONS": "AspNet,HttpClient",
+        "OTEL_DOTNET_AUTO_ENABLED_INSTRUMENTATIONS": "AspNet,HttpClient",
 
         "OTEL_TRACES_EXPORTER": "zipkin",
         "OTEL_EXPORTER_ZIPKIN_ENDPOINT": "http://localhost:9411/api/v2/spans"
@@ -41,7 +41,7 @@
 
         "OTEL_SERVICE_NAME": "Samples.StartupHook.AspNetCoreMvc31",
 
-        "OTEL_DOTNET_TRACER_INSTRUMENTATIONS": "AspNet,HttpClient",
+        "OTEL_DOTNET_AUTO_ENABLED_INSTRUMENTATIONS": "AspNet,HttpClient",
 
         "OTEL_TRACES_EXPORTER": "zipkin",
         "OTEL_EXPORTER_ZIPKIN_ENDPOINT": "http://localhost:9411/api/v2/spans"
diff --git a/src/OpenTelemetry.ClrProfiler.Managed.Loader/Startup.NetCore.cs b/src/OpenTelemetry.ClrProfiler.Managed.Loader/Startup.NetCore.cs
index f9852f7c8..3a9fe3a31 100644
--- a/src/OpenTelemetry.ClrProfiler.Managed.Loader/Startup.NetCore.cs
+++ b/src/OpenTelemetry.ClrProfiler.Managed.Loader/Startup.NetCore.cs
@@ -15,7 +15,7 @@ namespace OpenTelemetry.ClrProfiler.Managed.Loader
         private static string ResolveManagedProfilerDirectory()
         {
             string tracerFrameworkDirectory = "netcoreapp3.1";
-            string tracerHomeDirectory = ReadEnvironmentVariable("OTEL_DOTNET_TRACER_HOME") ?? string.Empty;
+            string tracerHomeDirectory = ReadEnvironmentVariable("OTEL_DOTNET_AUTO_HOME") ?? string.Empty;
 
             return Path.Combine(tracerHomeDirectory, tracerFrameworkDirectory);
         }
diff --git a/src/OpenTelemetry.ClrProfiler.Managed.Loader/Startup.NetFramework.cs b/src/OpenTelemetry.ClrProfiler.Managed.Loader/Startup.NetFramework.cs
index 2747656c8..61cda9eb1 100644
--- a/src/OpenTelemetry.ClrProfiler.Managed.Loader/Startup.NetFramework.cs
+++ b/src/OpenTelemetry.ClrProfiler.Managed.Loader/Startup.NetFramework.cs
@@ -13,7 +13,7 @@ namespace OpenTelemetry.ClrProfiler.Managed.Loader
     {
         private static string ResolveManagedProfilerDirectory()
         {
-            var tracerHomeDirectory = ReadEnvironmentVariable("OTEL_DOTNET_TRACER_HOME") ?? string.Empty;
+            var tracerHomeDirectory = ReadEnvironmentVariable("OTEL_DOTNET_AUTO_HOME") ?? string.Empty;
             var tracerFrameworkDirectory = "net461";
             return Path.Combine(tracerHomeDirectory, tracerFrameworkDirectory);
         }
diff --git a/src/OpenTelemetry.ClrProfiler.Managed.Loader/StartupLogger.cs b/src/OpenTelemetry.ClrProfiler.Managed.Loader/StartupLogger.cs
index fecb5fa46..c69ebee41 100644
--- a/src/OpenTelemetry.ClrProfiler.Managed.Loader/StartupLogger.cs
+++ b/src/OpenTelemetry.ClrProfiler.Managed.Loader/StartupLogger.cs
@@ -61,11 +61,11 @@ namespace OpenTelemetry.ClrProfiler.Managed.Loader
 
             try
             {
-                logDirectory = Environment.GetEnvironmentVariable("OTEL_TRACE_LOG_DIRECTORY");
+                logDirectory = Environment.GetEnvironmentVariable("OTEL_DOTNET_AUTO_LOG_DIRECTORY");
 
                 if (logDirectory == null)
                 {
-                    var nativeLogFile = Environment.GetEnvironmentVariable("OTEL_TRACE_LOG_PATH");
+                    var nativeLogFile = Environment.GetEnvironmentVariable("OTEL_DOTNET_AUTO_LOG_PATH");
 
                     if (!string.IsNullOrEmpty(nativeLogFile))
                     {
@@ -143,7 +143,7 @@ namespace OpenTelemetry.ClrProfiler.Managed.Loader
         {
             try
             {
-                var ddTraceDebugValue = Environment.GetEnvironmentVariable("OTEL_TRACE_DEBUG");
+                var ddTraceDebugValue = Environment.GetEnvironmentVariable("OTEL_DOTNET_AUTO_DEBUG");
 
                 if (ddTraceDebugValue == null)
                 {
diff --git a/src/OpenTelemetry.ClrProfiler.Managed/Configuration/ConfigurationKeys.cs b/src/OpenTelemetry.ClrProfiler.Managed/Configuration/ConfigurationKeys.cs
index 76e4a47e1..6a929b1c3 100644
--- a/src/OpenTelemetry.ClrProfiler.Managed/Configuration/ConfigurationKeys.cs
+++ b/src/OpenTelemetry.ClrProfiler.Managed/Configuration/ConfigurationKeys.cs
@@ -12,12 +12,12 @@ namespace OpenTelemetry.ClrProfiler.Managed.Configuration
         /// Default is value is true (enabled).
         /// </summary>
         /// <seealso cref="Settings.TraceEnabled"/>
-        public const string TraceEnabled = "OTEL_TRACE_ENABLED";
+        public const string TraceEnabled = "OTEL_DOTNET_AUTO_ENABLED";
 
         /// <summary>
         /// Configuration key for whether the tracer should be initialized by the profiler or not.
         /// </summary>
-        public const string LoadTracerAtStartup = "OTEL_DOTNET_TRACER_LOAD_AT_STARTUP";
+        public const string LoadTracerAtStartup = "OTEL_DOTNET_AUTO_LOAD_AT_STARTUP";
 
         /// <summary>
         /// Configuration key for the traces exporter to be used.
@@ -39,32 +39,32 @@ namespace OpenTelemetry.ClrProfiler.Managed.Configuration
         /// <summary>
         /// Configuration key for whether the console exporter is enabled.
         /// </summary>
-        public const string ConsoleExporterEnabled = "OTEL_DOTNET_TRACER_CONSOLE_EXPORTER_ENABLED";
+        public const string ConsoleExporterEnabled = "OTEL_DOTNET_AUTO_CONSOLE_EXPORTER_ENABLED";
 
         /// <summary>
         /// Configuration key for comma separated list of enabled instrumentations.
         /// </summary>
-        public const string Instrumentations = "OTEL_DOTNET_TRACER_INSTRUMENTATIONS";
+        public const string Instrumentations = "OTEL_DOTNET_AUTO_ENABLED_INSTRUMENTATIONS";
 
         /// <summary>
         /// Configuration key for comma separated list of disabled instrumentations.
         /// </summary>
-        public const string DisabledInstrumentations = "OTEL_DOTNET_TRACER_DISABLED_INSTRUMENTATIONS";
+        public const string DisabledInstrumentations = "OTEL_DOTNET_AUTO_DISABLED_INSTRUMENTATIONS";
 
         /// <summary>
         /// Configuration key for colon (:) separated list of plugins represented by <see cref="System.Type.AssemblyQualifiedName"/>.
         /// </summary>
-        public const string ProviderPlugins = "OTEL_DOTNET_TRACER_INSTRUMENTATION_PLUGINS";
+        public const string ProviderPlugins = "OTEL_DOTNET_AUTO_INSTRUMENTATION_PLUGINS";
 
         /// <summary>
         /// Configuration key for additional <see cref="ActivitySource"/> names to be added to the tracer at the startup.
         /// </summary>
-        public const string AdditionalSources = "OTEL_DOTNET_TRACER_ADDITIONAL_SOURCES";
+        public const string AdditionalSources = "OTEL_DOTNET_AUTO_ADDITIONAL_SOURCES";
 
         /// <summary>
         /// Configuration key for legacy source names to be added to the tracer at the startup.
         /// </summary>
-        public const string LegacySources = "OTEL_DOTNET_TRACER_LEGACY_SOURCES";
+        public const string LegacySources = "OTEL_DOTNET_AUTO_LEGACY_SOURCES";
 
         /// <summary>
         /// String format patterns used to match integration-specific configuration keys.
@@ -74,7 +74,7 @@ namespace OpenTelemetry.ClrProfiler.Managed.Configuration
             /// <summary>
             /// Configuration key pattern for enabling or disabling an integration.
             /// </summary>
-            public const string Enabled = "OTEL_TRACE_{0}_ENABLED";
+            public const string Enabled = "OTEL_DOTNET_AUTO_{0}_ENABLED";
         }
     }
 }
diff --git a/src/OpenTelemetry.ClrProfiler.Managed/Configuration/IntegrationSettings.cs b/src/OpenTelemetry.ClrProfiler.Managed/Configuration/IntegrationSettings.cs
index 419872554..09bf7e93f 100644
--- a/src/OpenTelemetry.ClrProfiler.Managed/Configuration/IntegrationSettings.cs
+++ b/src/OpenTelemetry.ClrProfiler.Managed/Configuration/IntegrationSettings.cs
@@ -21,8 +21,7 @@ namespace OpenTelemetry.ClrProfiler.Managed.Configuration
                 return;
             }
 
-            Enabled = source.GetBool(string.Format(ConfigurationKeys.Integrations.Enabled, integrationName)) ??
-                      source.GetBool(string.Format("OTEL_{0}_ENABLED", integrationName));
+            Enabled = source.GetBool(string.Format(ConfigurationKeys.Integrations.Enabled, integrationName));
         }
 
         /// <summary>
diff --git a/src/OpenTelemetry.ClrProfiler.Native/environment_variables.h b/src/OpenTelemetry.ClrProfiler.Native/environment_variables.h
index 2c5073d2e..975feeb9c 100644
--- a/src/OpenTelemetry.ClrProfiler.Native/environment_variables.h
+++ b/src/OpenTelemetry.ClrProfiler.Native/environment_variables.h
@@ -8,66 +8,66 @@ namespace environment {
 
 // Sets whether the profiler is enabled. Default is true.
 // Setting this to false disabled the profiler entirely.
-const WSTRING tracing_enabled = WStr("OTEL_TRACE_ENABLED");
+const WSTRING tracing_enabled = WStr("OTEL_DOTNET_AUTO_ENABLED");
 
 // Sets whether debug mode is enabled. Default is false.
-const WSTRING debug_enabled = WStr("OTEL_TRACE_DEBUG");
+const WSTRING debug_enabled = WStr("OTEL_DOTNET_AUTO_DEBUG");
 
 // Sets the paths to integration definition JSON files.
 // Supports multiple values separated with comma, for example:
 // "C:\Program Files\OpenTelemetry .NET AutoInstrumentation\integrations.json,D:\temp\test_integrations.json"
-const WSTRING integrations_path = WStr("OTEL_INTEGRATIONS");
+const WSTRING integrations_path = WStr("OTEL_DOTNET_AUTO_INTEGRATIONS_FILE");
 
 // Sets the path to the profiler's home directory, for example:
 // "C:\Program Files\OpenTelemetry .NET AutoInstrumentation\" or "/opt/opentelemetry/"
-const WSTRING profiler_home_path = WStr("OTEL_DOTNET_TRACER_HOME");
+const WSTRING profiler_home_path = WStr("OTEL_DOTNET_AUTO_HOME");
 
 // Sets the filename of executables the profiler can attach to.
 // If not defined (default), the profiler will attach to any process.
 // Supports multiple values separated with comma, for example:
 // "MyApp.exe,dotnet.exe"
-const WSTRING include_process_names = WStr("OTEL_PROFILER_PROCESSES");
+const WSTRING include_process_names = WStr("OTEL_DOTNET_AUTO_INCLUDE_PROCESSES");
 
 // Sets the filename of executables the profiler cannot attach to.
 // If not defined (default), the profiler will attach to any process.
 // Supports multiple values separated with comma, for example:
 // "MyApp.exe,dotnet.exe"
-const WSTRING exclude_process_names = WStr("OTEL_PROFILER_EXCLUDE_PROCESSES");
+const WSTRING exclude_process_names = WStr("OTEL_DOTNET_AUTO_EXCLUDE_PROCESSES");
 
 // Sets a list of integrations to disable. All other integrations will remain
 // enabled. If not set (default), all integrations are enabled. Supports
 // multiple values separated with comma, for example:
 // "ElasticsearchNet,AspNetWebApi2"
 const WSTRING disabled_integrations =
-    WStr("OTEL_DOTNET_TRACER_DISABLED_INSTRUMENTATIONS");
+    WStr("OTEL_DOTNET_AUTO_DISABLED_INSTRUMENTATIONS");
 
 // Sets the path for the profiler's log file.
-// Environment variable OTEL_TRACE_LOG_DIRECTORY takes precedence over this setting, if set.
-const WSTRING log_path = WStr("OTEL_TRACE_LOG_PATH");
+// Environment variable OTEL_DOTNET_AUTO_LOG_DIRECTORY takes precedence over this setting, if set.
+const WSTRING log_path = WStr("OTEL_DOTNET_AUTO_LOG_PATH");
 
 // Sets the directory for the profiler's log file.
-// If set, this setting takes precedence over environment variable OTEL_TRACE_LOG_PATH.
+// If set, this setting takes precedence over environment variable OTEL_DOTNET_AUTO_LOG_PATH.
 // If not set, default is
 // "%ProgramData%"\OpenTelemetry .NET AutoInstrumentation\logs\" on Windows or
 // "/var/log/opentelemetry/dotnet/" on Linux.
-const WSTRING log_directory = WStr("OTEL_TRACE_LOG_DIRECTORY");
+const WSTRING log_directory = WStr("OTEL_DOTNET_AUTO_LOG_DIRECTORY");
 
 // Sets whether to disable all JIT optimizations.
 // Default value is false (do not disable all optimizations).
 // https://github.com/dotnet/coreclr/issues/24676
 // https://github.com/dotnet/coreclr/issues/12468
-const WSTRING clr_disable_optimizations = WStr("OTEL_CLR_DISABLE_OPTIMIZATIONS");
+const WSTRING clr_disable_optimizations = WStr("OTEL_DOTNET_AUTO_CLR_DISABLE_OPTIMIZATIONS");
 
 // Sets whether to intercept method calls when the caller method is inside a
 // domain-neutral assembly. This is dangerous because the integration assembly
 // the user can guarantee that all Application Pools on the system have at most
 // Default is false. Only used in .NET Framework 4.5 and 4.5.1.
 // https://github.com/DataDog/dd-trace-dotnet/pull/671
-const WSTRING domain_neutral_instrumentation = WStr("OTEL_TRACE_DOMAIN_NEUTRAL_INSTRUMENTATION");
+const WSTRING domain_neutral_instrumentation = WStr("OTEL_DOTNET_AUTO_DOMAIN_NEUTRAL_INSTRUMENTATION");
 
 // Indicates whether the profiler is running in the context
 // of Azure App Services
-const WSTRING azure_app_services = WStr("OTEL_AZURE_APP_SERVICES");
+const WSTRING azure_app_services = WStr("OTEL_DOTNET_AUTO_AZURE_APP_SERVICES");
 
 // The app_pool_id in the context of azure app services
 const WSTRING azure_app_services_app_pool_id = WStr("APP_POOL_ID");
@@ -77,17 +77,17 @@ const WSTRING azure_app_services_cli_telemetry_profile_value =
     WStr("DOTNET_CLI_TELEMETRY_PROFILE");
 
 // Enable the profiler to dump the IL original code and modification to the log.
-const WSTRING dump_il_rewrite_enabled = WStr("OTEL_DUMP_ILREWRITE_ENABLED");
+const WSTRING dump_il_rewrite_enabled = WStr("OTEL_DOTNET_AUTO_DUMP_ILREWRITE_ENABLED");
 
 // Sets whether to enable JIT inlining
-const WSTRING clr_enable_inlining = WStr("OTEL_CLR_ENABLE_INLINING");
+const WSTRING clr_enable_inlining = WStr("OTEL_DOTNET_AUTO_CLR_ENABLE_INLINING");
 
 // Custom internal tracer profiler path
 const WSTRING internal_trace_profiler_path =
-    WStr("OTEL_INTERNAL_TRACE_NATIVE_ENGINE_PATH");
+    WStr("OTEL_DOTNET_AUTO_INTERNAL_NATIVE_ENGINE_PATH");
 
 // Sets whether to enable NGEN images.
-const WSTRING clr_enable_ngen = WStr("OTEL_CLR_ENABLE_NGEN");
+const WSTRING clr_enable_ngen = WStr("OTEL_DOTNET_AUTO_CLR_ENABLE_NGEN");
 
 // The list of startup hooks defined for .NET Core 3.1+ applications.
 // This is a .NET runtime environment variable. 
diff --git a/src/OpenTelemetry.Instrumentation.StartupHook/StartupHook.cs b/src/OpenTelemetry.Instrumentation.StartupHook/StartupHook.cs
index 267fa08d2..ce46e2eee 100644
--- a/src/OpenTelemetry.Instrumentation.StartupHook/StartupHook.cs
+++ b/src/OpenTelemetry.Instrumentation.StartupHook/StartupHook.cs
@@ -101,7 +101,7 @@ internal class StartupHook
     {
         var excludedProcesses = new List<string>();
 
-        var environmentValue = GetEnvironmentVariable("OTEL_PROFILER_EXCLUDE_PROCESSES");
+        var environmentValue = GetEnvironmentVariable("OTEL_DOTNET_AUTO_EXCLUDE_PROCESSES");
 
         if (environmentValue == null)
         {
diff --git a/test/OpenTelemetry.ClrProfiler.Managed.Bootstrapping.Tests/InstrumentationTests.cs b/test/OpenTelemetry.ClrProfiler.Managed.Bootstrapping.Tests/InstrumentationTests.cs
index c282fde91..26467b1e7 100644
--- a/test/OpenTelemetry.ClrProfiler.Managed.Bootstrapping.Tests/InstrumentationTests.cs
+++ b/test/OpenTelemetry.ClrProfiler.Managed.Bootstrapping.Tests/InstrumentationTests.cs
@@ -19,7 +19,7 @@ namespace OpenTelemetry.ClrProfiler.Managed.Bootstrapping.Tests
         [FactRequiringEnvVar]
         public void Initialize_WithDisabledFlag_DoesNotCreateTracerProvider()
         {
-            Environment.SetEnvironmentVariable("OTEL_DOTNET_TRACER_LOAD_AT_STARTUP", "false");
+            Environment.SetEnvironmentVariable("OTEL_DOTNET_AUTO_LOAD_AT_STARTUP", "false");
 
             Instrumentation.Initialize();
             var otelActivity = _otelActivitySource.StartActivity("OtelActivity");
@@ -45,7 +45,7 @@ namespace OpenTelemetry.ClrProfiler.Managed.Bootstrapping.Tests
         [FactRequiringEnvVar]
         public void Initialize_WithEnabledFlag_CreatesTracerProvider()
         {
-            Environment.SetEnvironmentVariable("OTEL_DOTNET_TRACER_LOAD_AT_STARTUP", "true");
+            Environment.SetEnvironmentVariable("OTEL_DOTNET_AUTO_LOAD_AT_STARTUP", "true");
 
             Instrumentation.Initialize();
             var otelActivity = _otelActivitySource.StartActivity("OtelActivity");
@@ -58,7 +58,7 @@ namespace OpenTelemetry.ClrProfiler.Managed.Bootstrapping.Tests
         [FactRequiringEnvVar]
         public void Initialize_WithPreviouslyCreatedTracerProvider_WorksCorrectly()
         {
-            Environment.SetEnvironmentVariable("OTEL_DOTNET_TRACER_LOAD_AT_STARTUP", "false");
+            Environment.SetEnvironmentVariable("OTEL_DOTNET_AUTO_LOAD_AT_STARTUP", "false");
             var tracer = Sdk
                 .CreateTracerProviderBuilder()
                 .AddSource("Custom")
diff --git a/test/OpenTelemetry.ClrProfiler.Managed.Loader.Tests/StartupTests.cs b/test/OpenTelemetry.ClrProfiler.Managed.Loader.Tests/StartupTests.cs
index d2901cb88..bc020aa03 100644
--- a/test/OpenTelemetry.ClrProfiler.Managed.Loader.Tests/StartupTests.cs
+++ b/test/OpenTelemetry.ClrProfiler.Managed.Loader.Tests/StartupTests.cs
@@ -11,7 +11,7 @@ namespace OpenTelemetry.ClrProfiler.Managed.Loader.Tests
         public void Ctor_LoadsManagedAssembly()
         {
             var directory = Directory.GetCurrentDirectory();
-            Environment.SetEnvironmentVariable("OTEL_DOTNET_TRACER_HOME", Path.Combine(directory, "..", "Profiler"));
+            Environment.SetEnvironmentVariable("OTEL_DOTNET_AUTO_HOME", Path.Combine(directory, "..", "Profiler"));
 
             var exception = Record.Exception(() => Startup.ManagedProfilerDirectory);
 
diff --git a/test/integration-tests/IntegrationTests.Helpers/EnvironmentHelper.cs b/test/integration-tests/IntegrationTests.Helpers/EnvironmentHelper.cs
index f82033479..660eb7eac 100644
--- a/test/integration-tests/IntegrationTests.Helpers/EnvironmentHelper.cs
+++ b/test/integration-tests/IntegrationTests.Helpers/EnvironmentHelper.cs
@@ -98,12 +98,12 @@ namespace IntegrationTests.Helpers
                 "COR_PROFILER_PATH",
 
                 // OpenTelemetry
-                "OTEL_PROFILER_PROCESSES",
-                "OTEL_DOTNET_TRACER_HOME",
-                "OTEL_INTEGRATIONS",
+                "OTEL_DOTNET_AUTO_INCLUDE_PROCESSES",
+                "OTEL_DOTNET_AUTO_HOME",
+                "OTEL_DOTNET_AUTO_INTEGRATIONS_FILE",
                 "OTEL_DISABLED_INTEGRATIONS",
-                "OTEL_DOTNET_TRACER_ADDITIONAL_SOURCES",
-                "OTEL_PROFILER_EXCLUDE_PROCESSES"
+                "OTEL_DOTNET_AUTO_ADDITIONAL_SOURCES",
+                "OTEL_DOTNET_AUTO_EXCLUDE_PROCESSES"
             };
 
             foreach (string variable in environmentVariables)
@@ -171,25 +171,25 @@ namespace IntegrationTests.Helpers
 
             if (DebugModeEnabled)
             {
-                environmentVariables["OTEL_TRACE_DEBUG"] = "1";
-                environmentVariables["OTEL_TRACE_LOG_DIRECTORY"] = Path.Combine(EnvironmentTools.GetSolutionDirectory(), "build_data", "profiler-logs");
+                environmentVariables["OTEL_DOTNET_AUTO_DEBUG"] = "1";
+                environmentVariables["OTEL_DOTNET_AUTO_LOG_DIRECTORY"] = Path.Combine(EnvironmentTools.GetSolutionDirectory(), "build_data", "profiler-logs");
             }
 
             if (!string.IsNullOrEmpty(processToProfile))
             {
-                environmentVariables["OTEL_PROFILER_PROCESSES"] = Path.GetFileName(processToProfile);
+                environmentVariables["OTEL_DOTNET_AUTO_INCLUDE_PROCESSES"] = Path.GetFileName(processToProfile);
             }
 
             string integrations = GetIntegrationsPath();
-            environmentVariables["OTEL_DOTNET_TRACER_HOME"] = GetNukeBuildOutput();
-            environmentVariables["OTEL_INTEGRATIONS"] = integrations;
+            environmentVariables["OTEL_DOTNET_AUTO_HOME"] = GetNukeBuildOutput();
+            environmentVariables["OTEL_DOTNET_AUTO_INTEGRATIONS_FILE"] = integrations;
             environmentVariables["OTEL_TRACES_EXPORTER"] = "zipkin";
             environmentVariables["OTEL_EXPORTER_ZIPKIN_ENDPOINT"] = $"http://127.0.0.1:{agentPort}";
 
             // for ASP.NET Core sample apps, set the server's port
             environmentVariables["ASPNETCORE_URLS"] = $"http://127.0.0.1:{aspNetCorePort}/";
 
-            environmentVariables["OTEL_DOTNET_TRACER_ADDITIONAL_SOURCES"] = "Samples.*";
+            environmentVariables["OTEL_DOTNET_AUTO_ADDITIONAL_SOURCES"] = "Samples.*";
 
             foreach (var key in CustomEnvironmentVariables.Keys)
             {
diff --git a/test/integration-tests/IntegrationTests.StartupHook/StartupHookTests.cs b/test/integration-tests/IntegrationTests.StartupHook/StartupHookTests.cs
index 858b68c28..96d46ca99 100644
--- a/test/integration-tests/IntegrationTests.StartupHook/StartupHookTests.cs
+++ b/test/integration-tests/IntegrationTests.StartupHook/StartupHookTests.cs
@@ -20,7 +20,7 @@ namespace IntegrationTests.StartupHook
             : base("StartupHook", output)
         {
             SetEnvironmentVariable("OTEL_SERVICE_NAME", ServiceName);
-            SetEnvironmentVariable("OTEL_DOTNET_TRACER_INSTRUMENTATIONS", "HttpClient");
+            SetEnvironmentVariable("OTEL_DOTNET_AUTO_ENABLED_INSTRUMENTATIONS", "HttpClient");
             _expectations.Add(new WebServerSpanExpectation(ServiceName, null, "SayHello", "SayHello", null));
             _expectations.Add(new WebServerSpanExpectation(ServiceName, null, "HTTP GET", "HTTP GET", null, "GET"));
         }
@@ -61,7 +61,7 @@ namespace IntegrationTests.StartupHook
                 expectedSpanCount = 0;
             }
 
-            SetEnvironmentVariable("OTEL_PROFILER_EXCLUDE_PROCESSES", $"dotnet,dotnet.exe,{applicationNameToExclude}");
+            SetEnvironmentVariable("OTEL_DOTNET_AUTO_EXCLUDE_PROCESSES", $"dotnet,dotnet.exe,{applicationNameToExclude}");
 
             var spans = RunTestApplication(enableStartupHook: true);
 
diff --git a/test/test-applications/integrations/Samples.GraphQL/Properties/launchSettings.json b/test/test-applications/integrations/Samples.GraphQL/Properties/launchSettings.json
index eb9788c7b..762ea31d1 100644
--- a/test/test-applications/integrations/Samples.GraphQL/Properties/launchSettings.json
+++ b/test/test-applications/integrations/Samples.GraphQL/Properties/launchSettings.json
@@ -19,11 +19,11 @@
         "CORECLR_PROFILER_PATH": "$(SolutionDir)bin\\tracer-home\\win-x64\\OpenTelemetry.ClrProfiler.Native.dll",
         "DOTNET_STARTUP_HOOKS": "$(SolutionDir)bin\\tracer-home\\netcoreapp3.1\\OpenTelemetry.Instrumentation.StartupHook.dll",
 
-        "OTEL_DOTNET_TRACER_HOME": "$(SolutionDir)bin\\tracer-home\\",
-        "OTEL_INTEGRATIONS": "$(SolutionDir)bin\\tracer-home\\integrations.json",
+        "OTEL_DOTNET_AUTO_HOME": "$(SolutionDir)bin\\tracer-home\\",
+        "OTEL_DOTNET_AUTO_INTEGRATIONS_FILE": "$(SolutionDir)bin\\tracer-home\\integrations.json",
         "OTEL_TRACES_EXPORTER": "zipkin",
         "OTEL_EXPORTER_ZIPKIN_ENDPOINT": "http://127.0.0.1:9411/api/v2/spans",
-        "OTEL_TRACE_DEBUG": "1"
+        "OTEL_DOTNET_AUTO_DEBUG": "1"
       },
       "applicationUrl": "http://localhost:54568/",
       "nativeDebugging": true
diff --git a/test/test-applications/integrations/Samples.MongoDB/Properties/launchSettings.json b/test/test-applications/integrations/Samples.MongoDB/Properties/launchSettings.json
index 91a281778..9fed8a8a5 100644
--- a/test/test-applications/integrations/Samples.MongoDB/Properties/launchSettings.json
+++ b/test/test-applications/integrations/Samples.MongoDB/Properties/launchSettings.json
@@ -8,11 +8,11 @@
         "CORECLR_PROFILER_PATH": "$(SolutionDir)bin\\tracer-home\\win-x64\\OpenTelemetry.ClrProfiler.Native.dll",
         "DOTNET_STARTUP_HOOKS": "$(SolutionDir)bin\\tracer-home\\netcoreapp3.1\\OpenTelemetry.Instrumentation.StartupHook.dll",
 
-        "OTEL_DOTNET_TRACER_HOME": "$(SolutionDir)bin\\tracer-home\\",
-        "OTEL_INTEGRATIONS": "$(SolutionDir)bin\\tracer-home\\integrations.json",
+        "OTEL_DOTNET_AUTO_HOME": "$(SolutionDir)bin\\tracer-home\\",
+        "OTEL_DOTNET_AUTO_INTEGRATIONS_FILE": "$(SolutionDir)bin\\tracer-home\\integrations.json",
         "OTEL_TRACES_EXPORTER": "zipkin",
         "OTEL_EXPORTER_ZIPKIN_ENDPOINT": "http://127.0.0.1:9411/api/v2/spans",
-        "OTEL_TRACE_DEBUG": "1"
+        "OTEL_DOTNET_AUTO_DEBUG": "1"
       }
     }
   }
diff --git a/test/test-applications/integrations/aspnet/Samples.AspNet/Dockerfile b/test/test-applications/integrations/aspnet/Samples.AspNet/Dockerfile
index b6b6773de..ad4b42352 100644
--- a/test/test-applications/integrations/aspnet/Samples.AspNet/Dockerfile
+++ b/test/test-applications/integrations/aspnet/Samples.AspNet/Dockerfile
@@ -4,15 +4,15 @@ ARG platform=x64
 ENV COR_ENABLE_PROFILING=1 \
     COR_PROFILER={918728DD-259F-4A6A-AC2B-B85E1B658318} \
     COR_PROFILER_PATH=C:\\opentelemetry\\win-${platform}\\OpenTelemetry.ClrProfiler.Native.dll \
-    OTEL_DOTNET_TRACER_HOME=C:\\opentelemetry\\ \
-    OTEL_INTEGRATIONS=C:\\opentelemetry\\integrations.json \
+    OTEL_DOTNET_AUTO_HOME=C:\\opentelemetry\\ \
+    OTEL_DOTNET_AUTO_INTEGRATIONS_FILE=C:\\opentelemetry\\integrations.json \
     OTEL_TRACES_EXPORTER=zipkin \
-    OTEL_DOTNET_TRACER_ADDITIONAL_SOURCES=Samples.* \
-    OTEL_DOTNET_TRACER_INSTRUMENTATIONS=HttpClient,AspNet \
+    OTEL_DOTNET_AUTO_ADDITIONAL_SOURCES=Samples.* \
+    OTEL_DOTNET_AUTO_ENABLED_INSTRUMENTATIONS=HttpClient,AspNet \
     OTEL_SERVICE_NAME=Samples.AspNet \
-    OTEL_TRACE_DOMAIN_NEUTRAL_INSTRUMENTATION=1 \
-    OTEL_TRACE_DEBUG=1 \
-    OTEL_TRACE_LOG_DIRECTORY=C:\\inetpub\\wwwroot\\logs \
-    OTEL_PROFILER_PROCESSES=w3wp.exe
+    OTEL_DOTNET_AUTO_DOMAIN_NEUTRAL_INSTRUMENTATION=1 \
+    OTEL_DOTNET_AUTO_DEBUG=1 \
+    OTEL_DOTNET_AUTO_LOG_DIRECTORY=C:\\inetpub\\wwwroot\\logs \
+    OTEL_DOTNET_AUTO_INCLUDE_PROCESSES=w3wp.exe
 WORKDIR C:\\inetpub\\wwwroot
 COPY bin/${configuration}/app.publish .