diff --git a/CHANGELOG.md b/CHANGELOG.md
index 578a158fcb..e6f4246618 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -1,6 +1,6 @@
 # Changelog
 
-## Unreleased
+## Version 2.20.1 (2025-09-19)
 
 ### 🛠️ Bug fixes
 
diff --git a/README.md b/README.md
index fd20606aef..e9dc37c3c1 100644
--- a/README.md
+++ b/README.md
@@ -33,7 +33,7 @@ If you are looking for documentation on using those.
 ## Getting Started
 
 Download
-the [latest version](https://github.com/open-telemetry/opentelemetry-java-instrumentation/releases/download/v2.20.0/opentelemetry-javaagent.jar).
+the [latest version](https://github.com/open-telemetry/opentelemetry-java-instrumentation/releases/download/v2.20.1/opentelemetry-javaagent.jar).
 
 This package includes the instrumentation agent as well as
 instrumentations for all supported libraries and all available data exporters.
diff --git a/docs/apidiffs/current_vs_latest/opentelemetry-instrumentation-annotations.txt b/docs/apidiffs/current_vs_latest/opentelemetry-instrumentation-annotations.txt
index 40665bc6db..0219bb0667 100644
--- a/docs/apidiffs/current_vs_latest/opentelemetry-instrumentation-annotations.txt
+++ b/docs/apidiffs/current_vs_latest/opentelemetry-instrumentation-annotations.txt
@@ -1,2 +1,2 @@
-Comparing source compatibility of opentelemetry-instrumentation-annotations-2.20.0.jar against opentelemetry-instrumentation-annotations-2.19.0.jar
+Comparing source compatibility of opentelemetry-instrumentation-annotations-2.20.1.jar against opentelemetry-instrumentation-annotations-2.19.0.jar
 No changes.
\ No newline at end of file
diff --git a/docs/apidiffs/current_vs_latest/opentelemetry-instrumentation-api.txt b/docs/apidiffs/current_vs_latest/opentelemetry-instrumentation-api.txt
index eb51d0dbf5..3912ebe43e 100644
--- a/docs/apidiffs/current_vs_latest/opentelemetry-instrumentation-api.txt
+++ b/docs/apidiffs/current_vs_latest/opentelemetry-instrumentation-api.txt
@@ -1,2 +1,2 @@
-Comparing source compatibility of opentelemetry-instrumentation-api-2.20.0.jar against opentelemetry-instrumentation-api-2.19.0.jar
+Comparing source compatibility of opentelemetry-instrumentation-api-2.20.1.jar against opentelemetry-instrumentation-api-2.19.0.jar
 No changes.
\ No newline at end of file
diff --git a/docs/apidiffs/current_vs_latest/opentelemetry-spring-boot-autoconfigure.txt b/docs/apidiffs/current_vs_latest/opentelemetry-spring-boot-autoconfigure.txt
index b0f1eba271..af7a8d23f6 100644
--- a/docs/apidiffs/current_vs_latest/opentelemetry-spring-boot-autoconfigure.txt
+++ b/docs/apidiffs/current_vs_latest/opentelemetry-spring-boot-autoconfigure.txt
@@ -1,2 +1,2 @@
-Comparing source compatibility of opentelemetry-spring-boot-autoconfigure-2.20.0.jar against opentelemetry-spring-boot-autoconfigure-2.19.0.jar
+Comparing source compatibility of opentelemetry-spring-boot-autoconfigure-2.20.1.jar against opentelemetry-spring-boot-autoconfigure-2.19.0.jar
 No changes.
\ No newline at end of file
diff --git a/docs/apidiffs/current_vs_latest/opentelemetry-spring-boot-starter.txt b/docs/apidiffs/current_vs_latest/opentelemetry-spring-boot-starter.txt
index 7945cc3cf0..4f94132bae 100644
--- a/docs/apidiffs/current_vs_latest/opentelemetry-spring-boot-starter.txt
+++ b/docs/apidiffs/current_vs_latest/opentelemetry-spring-boot-starter.txt
@@ -1,2 +1,2 @@
-Comparing source compatibility of opentelemetry-spring-boot-starter-2.20.0.jar against opentelemetry-spring-boot-starter-2.19.0.jar
+Comparing source compatibility of opentelemetry-spring-boot-starter-2.20.1.jar against opentelemetry-spring-boot-starter-2.19.0.jar
 No changes.
\ No newline at end of file
diff --git a/examples/distro/build.gradle b/examples/distro/build.gradle
index c54303404e..ce17fed12c 100644
--- a/examples/distro/build.gradle
+++ b/examples/distro/build.gradle
@@ -14,7 +14,7 @@ buildscript {
   dependencies {
     classpath "com.diffplug.spotless:spotless-plugin-gradle:7.2.1"
     classpath "com.gradleup.shadow:shadow-gradle-plugin:9.1.0"
-    classpath "io.opentelemetry.instrumentation:gradle-plugins:2.20.0-alpha"
+    classpath "io.opentelemetry.instrumentation:gradle-plugins:2.20.1-alpha"
   }
 }
 
@@ -30,8 +30,8 @@ subprojects {
       opentelemetrySdk           : "1.54.1",
 
       // these lines are managed by .github/scripts/update-version.sh
-      opentelemetryJavaagent     : "2.20.0",
-      opentelemetryJavaagentAlpha: "2.20.0-alpha",
+      opentelemetryJavaagent     : "2.20.1",
+      opentelemetryJavaagentAlpha: "2.20.1-alpha",
 
       autoservice                : "1.1.1"
     ]
diff --git a/examples/extension/build.gradle b/examples/extension/build.gradle
index 629003e165..d32d51f193 100644
--- a/examples/extension/build.gradle
+++ b/examples/extension/build.gradle
@@ -13,8 +13,8 @@ plugins {
   id "com.gradleup.shadow" version "9.1.0"
   id "com.diffplug.spotless" version "7.2.1"
 
-  id "io.opentelemetry.instrumentation.muzzle-generation" version "2.20.0-alpha"
-  id "io.opentelemetry.instrumentation.muzzle-check" version "2.20.0-alpha"
+  id "io.opentelemetry.instrumentation.muzzle-generation" version "2.20.1-alpha"
+  id "io.opentelemetry.instrumentation.muzzle-check" version "2.20.1-alpha"
 }
 
 group 'io.opentelemetry.example'
@@ -26,8 +26,8 @@ ext {
     opentelemetrySdk           : "1.54.1",
 
     // these lines are managed by .github/scripts/update-version.sh
-    opentelemetryJavaagent     : "2.20.0",
-    opentelemetryJavaagentAlpha: "2.20.0-alpha"
+    opentelemetryJavaagent     : "2.20.1",
+    opentelemetryJavaagentAlpha: "2.20.1-alpha"
   ]
 
   deps = [
diff --git a/version.gradle.kts b/version.gradle.kts
index d174793c0d..282db803a9 100644
--- a/version.gradle.kts
+++ b/version.gradle.kts
@@ -1,5 +1,5 @@
-val stableVersion = "2.20.0"
-val alphaVersion = "2.20.0-alpha"
+val stableVersion = "2.20.1"
+val alphaVersion = "2.20.1-alpha"
 
 allprojects {
   if (findProperty("otel.stable") != "true") {