diff --git a/tox.ini b/tox.ini
index 3cec9fcfb..978155616 100644
--- a/tox.ini
+++ b/tox.ini
@@ -36,6 +36,10 @@ envlist =
     py3{4,5,6,7,8}-test-ext-dbapi
     pypy3-test-ext-dbapi
 
+    ; opentelemetry-ext-boto
+    py3{5,6,7,8}-test-ext-boto
+    pypy3-test-ext-boto
+
     ; opentelemetry-ext-flask
     py3{4,5,6,7,8}-test-ext-flask
     pypy3-test-ext-flask
@@ -161,6 +165,7 @@ changedir =
   test-ext-sqlite3: ext/opentelemetry-ext-sqlite3/tests
   test-ext-wsgi: ext/opentelemetry-ext-wsgi/tests
   test-ext-zipkin: ext/opentelemetry-ext-zipkin/tests
+  test-ext-boto: ext/opentelemetry-ext-boto/tests
   test-ext-flask: ext/opentelemetry-ext-flask/tests
   test-example-app: docs/examples/opentelemetry-example-app/tests
   test-getting-started: docs/getting_started/tests
@@ -188,6 +193,10 @@ commands_pre =
   wsgi,flask,django: pip install {toxinidir}/ext/opentelemetry-ext-wsgi
   flask,django: pip install {toxinidir}/opentelemetry-auto-instrumentation
   asgi: pip install {toxinidir}/ext/opentelemetry-ext-asgi
+
+  boto: pip install {toxinidir}/opentelemetry-auto-instrumentation
+  boto: pip install {toxinidir}/ext/opentelemetry-ext-boto[test]
+
   flask: pip install {toxinidir}/ext/opentelemetry-ext-flask[test]
 
   dbapi: pip install {toxinidir}/ext/opentelemetry-ext-dbapi[test]