diff --git a/tox.ini b/tox.ini
index 384c43725..b477d5ba7 100644
--- a/tox.ini
+++ b/tox.ini
@@ -169,6 +169,11 @@ envlist =
     ; instrumentation-system-metrics intentionally excluded from pypy3
     ; known limitation: gc.get_count won't work under pypy
 
+    ; opentelemetry-instrumentation-tornado
+    ; instrumentation supports >=6 on Py 3.5 and above.
+    py3{5,6,7,8}-test-instrumentation-tornado
+    pypy3-test-instrumentation-tornado
+
     lint
     py38-tracecontext
     py38-{mypy,mypyinstalled}
@@ -229,6 +234,7 @@ changedir =
   test-instrumentation-sqlite3: instrumentation/opentelemetry-instrumentation-sqlite3/tests
   test-instrumentation-starlette: instrumentation/opentelemetry-instrumentation-starlette/tests
   test-instrumentation-system-metrics: instrumentation/opentelemetry-instrumentation-system-metrics/tests
+  test-instrumentation-tornado: instrumentation/opentelemetry-instrumentation-tornado/tests
   test-instrumentation-wsgi: instrumentation/opentelemetry-instrumentation-wsgi/tests
 
   test-exporter-jaeger: exporter/opentelemetry-exporter-jaeger/tests
@@ -303,6 +309,8 @@ commands_pre =
 
   starlette: pip install {toxinidir}/instrumentation/opentelemetry-instrumentation-starlette[test]
 
+  tornado: pip install {toxinidir}/instrumentation/opentelemetry-instrumentation-tornado
+
   jinja2: pip install {toxinidir}/instrumentation/opentelemetry-instrumentation-jinja2[test]
 
   aiohttp-client: pip install {toxinidir}/opentelemetry-sdk {toxinidir}/instrumentation/opentelemetry-instrumentation-aiohttp-client